Transmission Electron Microscopy

Basic Setup
Uses electrons, which have wavelengths of 0.1nm - 0.2nm, which effectively gives them a higher resolution, about 1000 times better than ou​r lab microscopes.

A condenser magnet is used instead of glass lenses to distort the electromagnetic waves; glass lenses would insulate the electrons.

A projector magnet is used to project it onto a fluorescent screen because electrons are highly ionizing and would damage the human eye.

Electron microscopy takes place in a vacuum so that electrons can flow through; air would interfere with the flow of electrons

The resolution is 0.5nm or 5 angstroms (5 Å), which is extremely high.

Appearance of Field and Object
With nothing in the field of view, it will be very bright and white



The less dense the material, the less you see; the more dense the material, the more you see because there will be more electrons that will scatter, and so the material will appear darker

Use
Used to see smaller things than normal microscopes can see, like viruses and inclusions of bacteria. TEM can even see large molecules such as DNA and some proteins